ABSTRACT
Harmine, Harmaline, Nicotine and its various complexes synthesized have been characterized by physical, spectral and analytical methods and curtained for in-vitro antimicrobial activity against different bacterial and fungal species at two different concentrations i.e.100micro g/100micro l and 200micro g/100micro l dose level respectively. Analysis showed that Nicotine, Zinc-Nico, Cd-Nico, Hg-Nico, Ni-Nico, Cu-Nico, Co-Nico, Harmine, and Harmaline having conc. of 100ug/ 100ul had antibacterial activity on zero, 5, 4, 10, zero, 5, 7, zero, zero strain of bacteria having an average of zero [SD=0.0000], 15.2000 [SD=1.30384], 18.2500 [SD=3.30404], 20.2000 [SD=1.39841], zero [SD=0.0000], 14.6000 [SD=0.89443], 15.8571 [SD=1.34519], zero [SD=0.0000], zero [SD=0.0000] respectively. Zinc [II] chloride, Cadmium [II] Iodide, Mercury [II] chloride, Nickel [II] chloride, Copper [II] chloride, Cobalt [II] chloride, Mercury [II] chloride, Mercury [II] harmine, Mercury [II] harmaline at 100ug/100ul is valid for 7, 8, 9, 2, 7, 8, 9, 10, 8 strains of bacteria with an average of 7.1429 [SD=1.06904], 10.0000 [SD=5.01427], 14.8889 [SD=6.00925], 6.0000 [SD=0.0000], 8.5714 [SD=4.27618], 8.2500 [SD=0.88641], 14.8889 [SD=6.00925], 18.6000 [SD=2.45855], 18.5000 [SD=1.85164] respectively. The above given compounds at the conc. of 200 micro g/100ul is valid for 10, 9, 10, 8, 8, 10, 10, 10, 10 strains of bacteria with an average of 8.1 [SD=1.66333], 11.7778 [SD=5.28625], 16.1000 [SD=6.36745], 6.5000 [SD=0.92582], 9.7500 [SD=4.43203], 9.9000 [SD=2.76687], 16.1000 [SD=6.36745], 22.0000 [SD=2.44949], 20.4000 [SD=2.75681] respectively. The above given compounds at conc. of 200 micro g/100ul showed antibacterial action on 3, 8, 8, 10, 3, 9, 8, zero, 3 strains of bacteria with an average of 14[SD=0.000], 16.8750 [SD=1.35620], 18.2500 [SD=3.45378], 22.7000 [SD=1.82878], 14.3333 [SD=0.57735], 16.7778 [SD=1.71594], zero [SD=0.000], 12.0000 [SD=1.00000] respectively. Hence according to the average value of the zone of inhibition, maximum antibacterial activity at 100-200ug/100ul is of Hg-Nico and Mercury salt; Mercury [II] harmine having an average of 20.2000 [SD=1.39841]-22.7000 [SD=1.82878] and 18.6000 [SD=2.45855]-22.0000 [SD=2.44949]. Minimum antibacterial activity at 100-200ug/100ul is Nicotine100, Nicotine-Nico100, Harmine 100,Harmaline 100, Harmine 200 having zero average [SD=0.000]